SIREM technician programing robot used to assemble Gene-Trac® qPCR reactions

Heat maps and other graphical outputs provide easy to interpret microbial community profiles in Gene-Trac® NGS reports

Gene-Trac® testing is used to quantify key microorganisms and to determine microbial community composition for the assessment of bioremediation potential and to monitor enhanced bioremediation performance by quantifying and characterizing microorganisms in groundwater and soil/sediment from contaminated sites.

Use Gene-Trac® Testing to:

  • Determine if suitable microorganisms are present for MNA remedies.
  • Predict the effectiveness of biostimulation before the addition of electron donor.
  • Determine the need for bioaugmentation.
  • Determine the impact of site amendments including, electron donors/acceptors.
  • Predict if intermediates such as cDCE or vinyl chloride are likely to accumulate.
  • Characterize microbial community spatial and temporal variability.
  • Validate the performance of enhanced bioremediation projects.
  • Make informed decisions to manage bioremediation and other microbial systems.

Quantify Environmental Microorganisms and Microbial Communities

SiREM offers a growing number of targets for Gene-Trac® testing that includes: Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg), Desulfitobacterium (Dsb) and key functional genes such as vinyl chloride reductase (vcrA, bvcA), trichloroethene reductase (tceA), chloroform, 1,1,1-TCA and 1,1-DCA reductase (cfrA/dcrA). These microorganisms and functional genes are critical to reductive dechlorination of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes, and propanes. Geobacter and sulfate reducers play key roles in the metabolism of aromatic hydrocarbon compounds and the generation of iron sulfur compounds critical to natural attenuation remedies. The dxmB and ALDH genes are important in the aerobic degradation of 1,4-dioxane.

Gene-Trac® quantitative polymerase chain reaction (qPCR) testing is used to quantify key dechlorinating bacteria and functional genes. If a comprehensive analysis of microbial community is required Gene-Trac® NGS (next generation sequencing) provides detailed microbial community composition to better understand the current and potential functions of microbial communities.

Available Gene-Trac® Tests Include

Test Target Relevance
Dhc Dehalococcoides Dechlorination of chlorinated ethenes, 1,2-DCA, PCB’s, TCB/DCB
FGA (vcrA/bvcA/tceA) VC-reductases/TCE-reductase Dhc functional genes: convert TCE, to cDCE and cDCE and VC to ethene
Dhb Dehalobacter Chlorinated methane and ethane degradation
cfrA cfrA and dcrA reductive dehalogenases Dhb functional genes: dechlorinate CF, 1,1,1-TCA and 1,1-DCA
Dhg Dehalogenimonas Dechlorinates chlorinated propanes, tDCE and 1,2-DCA
Dsb Desulfitobacterium Dechlorinates PCP, TCP/DCP, PCE/TCE, 1,2-DCA
Geo Geobacter Dechlorinates PCE and TCE/biogeochemical reduction
Polaromonas Polaromonas Isocitrate Lyase Aerobic cDCE/VC degraders (JS-666)
etn etnC/etnE Aerobic VC degradation
1,4-Dioxane dxmB-dioxane monooxygenase and ALDH aldehyde dehydrogenase Aerobic 1,4-Dioxane degradation
ORM-2 Deltaproteobacterium ORM-2 Anaerobic benzene degradation
Anammox Planctomycetes Ammonium and nitrite removal
SRB Sulfate reducing bacteria Anaerobic hydrocarbon oxidation/biogeochemical reduction
Arch Archaea Methanogenic microorganisms
Universal All bacteria For determining total bacterial biomass
NGS Bacteria and Archaea Next generation sequencing for overall microbial community composition

 

Gene-Trac® Features

  • Comprehensive reports provide detailed testing and quality control parameters
  • Electronic data deliverables in various formats available
  • USA or Canada sample reception options available
  • Field filter protocols are available that reduce shipping costs
  • Unparalleled technical support for data interpretation and planning

Use our on line form or contact SiREM to place an order for sampling supplies or to learn more about how Gene-Trac® can enhance your site monitoring and remediation program

pdf For more information on Gene-Trac® Testing

pdf For more information on NGS